4mag

Crystal structure of the Periplasmic Sialic Acid Binding Protein from Vibrio Cholerea

spinqualitylabelsall models 4mag, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4mag is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CO, SO4 Related: 3b50, 2cey, 2cex, 4mmp Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Sialic acids are a family of related nine-carbon sugar acids that play important roles in both eukaryotes and prokaryotes. These sialic acids are incorporated/decorated onto lipooligosaccharides as terminal sugars in multiple bacteria to evade the host immune system. Many pathogenic bacteria scavenge sialic acids from their host and use them for molecular mimicry. The first step of this process is the transport of sialic acid to the cytoplasm, which often takes place using a tripartite ATP-independent transport system consisting of a periplasmic binding protein and a membrane transporter. In this paper, the structural characterization of periplasmic binding proteins from the pathogenic bacteria Fusobacterium nucleatum, Pasteurella multocida and Vibrio cholerae and their thermodynamic characterization are reported. The binding affinities of several mutations in the Neu5Ac binding site of the Haemophilus influenzae protein are also reported. The structure and the thermodynamics of the binding of sugars suggest that all of these proteins have a very well conserved binding pocket and similar binding affinities. A significant conformational change occurs when these proteins bind the sugar. While the C1 carboxylate has been identified as the primary binding site, a second conserved hydrogen-bonding network is involved in the initiation and stabilization of the conformational states. Bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site.,Gangi Setty T, Cho C, Govindappa S, Apicella MA, Ramaswamy S Acta Crystallogr D Biol Crystallogr. 2014 Jul;70(Pt 7):1801-11. doi:, 10.1107/S139900471400830X. Epub 2014 Jun 24. PMID:25004958 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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