1v1f: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
The Arabidopsis thaliana SOS3 gene encodes a calcium sensor that is, required for plant salt tolerance. The SOS3 protein binds to and activates, the self-inhibited SOS2 protein kinase, which mediates the expression and, activities of various transporters important for ion homeostasis under, salt stress. SOS3 belongs to a unique family of calcium-binding proteins, that contain two pairs of EF hand motifs with four putative metal-binding, sites. We report the crystal structure of a dimeric SOS3 protein in, complex with calcium, and with calcium and manganese. Analytical, ultracentrifugation experiments and circular dichroism measurements show, that calcium binding is responsible for the dimerization of SOS3. This, leads to a change in the global shape and surface properties of the, protein | The Arabidopsis thaliana SOS3 gene encodes a calcium sensor that is, required for plant salt tolerance. The SOS3 protein binds to and activates, the self-inhibited SOS2 protein kinase, which mediates the expression and, activities of various transporters important for ion homeostasis under, salt stress. SOS3 belongs to a unique family of calcium-binding proteins, that contain two pairs of EF hand motifs with four putative metal-binding, sites. We report the crystal structure of a dimeric SOS3 protein in, complex with calcium, and with calcium and manganese. Analytical, ultracentrifugation experiments and circular dichroism measurements show, that calcium binding is responsible for the dimerization of SOS3. This, leads to a change in the global shape and surface properties of the, protein that may be sufficient to transmit the Ca(2+) signal elicited, during salt stress. | ||
==About this Structure== | ==About this Structure== | ||
1V1F is a | 1V1F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with CA, MN, IOD and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: EF1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V1F OCA]. | ||
==Reference== | ==Reference== | ||
| Line 29: | Line 29: | ||
[[Category: signalling protein]] | [[Category: signalling protein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:06:29 2007'' | ||
