2oqc: Difference between revisions
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[[Image:2oqc.jpg|left|200px]] | [[Image:2oqc.jpg|left|200px]] | ||
'''Crystal Structure of Penicillin V acylase from Bacillus subtilis''' | {{Structure | ||
|PDB= 2oqc |SIZE=350|CAPTION= <scene name='initialview01'>2oqc</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] | |||
|GENE= yxeI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |||
}} | |||
'''Crystal Structure of Penicillin V acylase from Bacillus subtilis''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2OQC is a [ | 2OQC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA]. | ||
==Reference== | ==Reference== | ||
Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http:// | Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511127 16511127] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Penicillin amidase]] | [[Category: Penicillin amidase]] | ||
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[[Category: Sivaraman, H.]] | [[Category: Sivaraman, H.]] | ||
[[Category: Suresh, C G.]] | [[Category: Suresh, C G.]] | ||
[[Category: bacillus | [[Category: bacillus subtili]] | ||
[[Category: choloylglycine hydrolase]] | [[Category: choloylglycine hydrolase]] | ||
[[Category: conjugated bile acid hydrolase]] | [[Category: conjugated bile acid hydrolase]] | ||
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[[Category: penicillin v acylase]] | [[Category: penicillin v acylase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:03:07 2008'' | ||
Revision as of 16:03, 20 March 2008
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| 2oqc, resolution 2.50Å | |||||||||||||
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| Gene: | yxeI (Bacillus subtilis) | ||||||||||||
| Activity: | Penicillin amidase, with EC number 3.5.1.11 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of Penicillin V acylase from Bacillus subtilis
Overview
Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3 Da(-1), corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model.
About this Structure
2OQC is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:16511127
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