2y6i

CRYSTAL STRUCTURE OF COLLAGENASE G FROM CLOSTRIDIUM HISTOLYTICUM IN COMPLEX WITH ISOAMYLPHOSPHONYL-GLY-PRO-ALA AT 3.25 ANGSTROM RESOLUTION

spinqualitylabelsall models 2y6i, resolution 3.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2y6i is a 2 chain structure with sequence from Clostridium histolyticum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 1PE, FLC, PEG, TRS, ZN NonStd Res: IPI Related: 2y50, 2y3u Activity: Microbial collagenase, with EC number 3.4.24.3 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Collagen constitutes one-third of body protein in humans, reflecting its extensive role in health and disease. Of similar importance, therefore, are the idiosyncratic proteases that have evolved for collagen remodeling. The most efficient collagenases are those that enable clostridial bacteria to colonize their host tissues; but despite intense study, the structural and mechanistic basis of these enzymes has remained elusive. Here we present the crystal structure of collagenase G from Clostridium histolyticum at 2.55-A resolution. By combining the structural data with enzymatic and mutagenesis studies, we derive a conformational two-state model of bacterial collagenolysis, in which recognition and unraveling of collagen microfibrils into triple helices, as well as unwinding of the triple helices, are driven by collagenase opening and closing. Structure of collagenase G reveals a chew-and-digest mechanism of bacterial collagenolysis.,Eckhard U, Schonauer E, Nuss D, Brandstetter H Nat Struct Mol Biol. 2011 Sep 25;18(10):1109-14. doi: 10.1038/nsmb.2127. PMID:21947205 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Collagenase (non-MMP)

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