4qa8

Crystal structure of LprF from Mycobacterium bovis

spinqualitylabelsall models 4qa8, resolution 1.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4qa8 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PJZ Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The Gram-positive bacteria Mycobacterium tuberculosis and M. bovis are causative agents of tuberculosis in humans and cattle. The lipoprotein LprF is found in M. tuberculosis and M. bovis but not in the nonpathogenic M. smegmatis. To date, the role of LprF remains to be elucidated. In this study, the crystal structure of LprF has been determined at 1.1 A resolution. The overall structure is similar to that of a homologue, LprG, with a central hydrophobic cavity that binds a triacylated glycolipid. LprF exhibited a central cavity structure similar to that of LprG, but with a smaller cavity that binds two alkyl chains. Consistently, subsequent mass-spectrometric analysis revealed that the bound ligand was a diacylated glycolipid, as found in the structure. Furthermore, an increased ratio of lipoarabinomannan to lipomannan in the mycobacterial cell wall was observed when lprF was introduced into M. smegmatis. These observations suggested that LprF transfers the diacylated glycolipid from the plasma membrane to the cell wall, which might be related to the pathogenesis of the bacteria. Crystal structure and functional implications of LprF from Mycobacterium tuberculosis and M. bovis.,Kim JS, Jiao L, Oh JI, Ha NC, Kim YH Acta Crystallogr D Biol Crystallogr. 2014 Oct 1;70(Pt 10):2619-30. doi:, 10.1107/S1399004714016599. Epub 2014 Sep 27. PMID:25286846 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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