4fnj: Difference between revisions
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[[ | ==Utilizing the GAAA tetraloop/receptor to facilitate crystal packing and structure determination of a CUG RNA helix== | ||
<StructureSection load='4fnj' size='340' side='right' caption='[[4fnj]], [[Resolution|resolution]] 1.95Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4fnj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FNJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FNJ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fnj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fnj RCSB], [http://www.ebi.ac.uk/pdbsum/4fnj PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Myotonic dystrophy type 1 (DM1) is a microsatellite expansion disorder caused by the aberrant expansion of CTG repeats in the 3'-untranslated region of the DMPK gene. When transcribed, the toxic RNA CUG repeats sequester RNA binding proteins, which leads to disease symptoms. The expanded CUG repeats can adopt a double-stranded structure, and targeting this helix is a therapeutic strategy for DM1. To improve our understanding of the 5'CUG/3'GUC motif and how it may interact with proteins and small molecules, we designed a short CUG helix attached to a GAAA tetraloop/receptor to facilitate crystal packing. Here we report the highest-resolution structure (1.95 A) to date of a GAAA tetraloop/receptor and the CUG helix it was used to crystallize. Within the CUG helix, we identify two different forms of noncanonical U-U pairs and reconfirm that CUG repeats are essentially A-form. An analysis of all noncanonical U-U pairs in the context of CUG repeats revealed six different classes of conformations that the noncanonical U-U pairs are able to adopt. | |||
Utilizing the GAAA Tetraloop/Receptor To Facilitate Crystal Packing and Determination of the Structure of a CUG RNA Helix.,Coonrod LA, Lohman JR, Berglund JA Biochemistry. 2012 Oct 12. PMID:23025897<ref>PMID:23025897</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Berglund, J A | [[Category: Berglund, J A]] | ||
[[Category: Coonrod, L A | [[Category: Coonrod, L A]] | ||
[[Category: Lohman, J R | [[Category: Lohman, J R]] | ||
[[Category: Cug repeat gaaa tetraloop/receptor]] | [[Category: Cug repeat gaaa tetraloop/receptor]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
[[Category: Toxic rna]] | [[Category: Toxic rna]] | ||
Revision as of 09:00, 25 January 2015
Utilizing the GAAA tetraloop/receptor to facilitate crystal packing and structure determination of a CUG RNA helix
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