3aq2

Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b

spinqualitylabelsall models 3aq2, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3aq2 is a 2 chain structure with sequence from Agrobacterium vitis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3aq3, 3aq4 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 A, Agrobacterium vitis AB6b at 1.65 A, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 A. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity. Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b.,Wang M, Soyano T, Machida S, Yang JY, Jung C, Chua NH, Yuan YA Genes Dev. 2011 Jan 1;25(1):64-76. Epub 2010 Dec 14. PMID:21156810 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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