3d0x

Crystal Structure of the unbound lysine riboswitch

spinqualitylabelsall models 3d0x, resolution 2.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3d0x is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3d0u Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Riboswitches are metabolite-sensitive elements found in mRNAs that control gene expression through a regulatory secondary structural switch. Along with regulation of lysine biosynthetic genes, mutations within the lysine-responsive riboswitch (L-box) play a role in the acquisition of resistance to antimicrobial lysine analogs. To understand the structural basis for lysine binding, we have determined the 2.8 angstroms resolution crystal structure of lysine bound to the Thermotoga maritima asd lysine riboswitch ligand-binding domain. The structure reveals a complex architecture scaffolding a binding pocket completely enveloping lysine. Mutations conferring antimicrobial resistance cluster around this site as well as highly conserved long range interactions, indicating that they disrupt lysine binding or proper folding of the RNA. Comparison of the free and bound forms by x-ray crystallography, small angle x-ray scattering, and chemical probing reveals almost identical structures, indicating that lysine induces only limited and local conformational changes upon binding. Crystal structure of the lysine riboswitch regulatory mRNA element.,Garst AD, Heroux A, Rambo RP, Batey RT J Biol Chem. 2008 Aug 15;283(33):22347-51. Epub 2008 Jul 1. PMID:18593706 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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