3bwp

Crystal structure of a self-spliced group II intron

spinqualitylabelsall models 3bwp, resolution 3.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3bwp is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: K, MG Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor. Crystal structure of a self-spliced group II intron.,Toor N, Keating KS, Taylor SD, Pyle AM Science. 2008 Apr 4;320(5872):77-82. PMID:18388288 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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