3c2j

Crystal structure analysis of trioxacarcin A covalently bound to d(AACCGGTT)

spinqualitylabelsall models 3c2j, resolution 1.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3c2j is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GGT Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed We report a crystal structure that shows an antibiotic that extracts a nucleobase from a DNA molecule 'caught in the act' after forming a covalent bond but before departing with the base. The structure of trioxacarcin A covalently bound to double-stranded d(AACCGGTT) was determined to 1.78 A resolution by MAD phasing employing brominated oligonucleotides. The DNA-drug complex has a unique structure that combines alkylation (at the N7 position of a guanine), intercalation (on the 3'-side of the alkylated guanine), and base flip-out. An antibiotic-induced flipping-out of a single, nonterminal nucleobase from a DNA duplex was observed for the first time in a crystal structure. Crystal structure of trioxacarcin A covalently bound to DNA.,Pfoh R, Laatsch H, Sheldrick GM Nucleic Acids Res. 2008 May 3;. PMID:18453630 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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