Odorant binding protein: Difference between revisions

Line 59:

*'''Activation by the pheromone alone'''

This model is supported by the pH dependent conformation transition, that is described above. The bulk of the sensillar lymph is in neutral pH (6.5-7), while environment near the dendrite membrane bears a low pH (4.5), due to the negative charges on the surface of the membrane <ref>DOI: 10.1016/0040-8166(84)90004-1</ref>, which cause the accumulation of positively charged kations near the membrane surface (20-50 nm)<ref name="kaissling" /~~><ref~~>. According to this model, the pheromone is entering the sensillar lymph through a pore in the cuticle, then it can be either degraded by odorant degrading enzymes (ODE) or bind to a PBP (of both forms). Once the complex is arriving to the low pH environment near the dendrite membrane the PBP will shift to the A-form, thereby ejaculating the ligand from the binding pocket, allowing it to activate the OR:CO-OR complex and the cellular signal transduction begins.

This model is supported by the pH dependent conformation transition, that is described above. The bulk of the sensillar lymph is in neutral pH (6.5-7), while environment near the dendrite membrane bears a low pH (4.5), due to the negative charges on the surface of the membrane <ref>DOI: 10.1016/0040-8166(84)90004-1</ref>, which cause the accumulation of positively charged kations near the membrane surface (20-50 nm)<ref name="kaissling" />. According to this model, the pheromone is entering the sensillar lymph through a pore in the cuticle, then it can be either degraded by odorant degrading enzymes (ODE) or bind to a PBP (of both forms). Once the complex is arriving to the low pH environment near the dendrite membrane the PBP will shift to the A-form, thereby ejaculating the ligand from the binding pocket, allowing it to activate the OR:CO-OR complex and the cellular signal transduction begins.

*'''Activation by the complex pheromone-PBP'''

Odorant binding protein: Difference between revisions

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@@ Line 59: / Line 59: @@
 *'''Activation by the pheromone alone'''
-This model is supported by the pH dependent conformation transition, that is described above. The bulk of the sensillar lymph is in neutral pH (6.5-7), while environment near the dendrite membrane bears a low pH (4.5), due to the negative charges on the surface of the membrane <ref>DOI: 10.1016/0040-8166(84)90004-1</ref>, which cause the accumulation of positively charged kations near the membrane surface (20-50 nm)<ref name="kaissling" /><ref>. According to this model, the pheromone is entering the sensillar lymph through a pore in the cuticle, then it can be either degraded by odorant degrading enzymes (ODE) or bind to a PBP (of both forms). Once the complex is arriving to the low pH environment near the dendrite membrane the PBP will shift to the A-form, thereby ejaculating the ligand from the binding pocket, allowing it to activate the OR:CO-OR complex and the cellular signal transduction begins.
+This model is supported by the pH dependent conformation transition, that is described above. The bulk of the sensillar lymph is in neutral pH (6.5-7), while environment near the dendrite membrane bears a low pH (4.5), due to the negative charges on the surface of the membrane <ref>DOI: 10.1016/0040-8166(84)90004-1</ref>, which cause the accumulation of positively charged kations near the membrane surface (20-50 nm)<ref name="kaissling" />. According to this model, the pheromone is entering the sensillar lymph through a pore in the cuticle, then it can be either degraded by odorant degrading enzymes (ODE) or bind to a PBP (of both forms). Once the complex is arriving to the low pH environment near the dendrite membrane the PBP will shift to the A-form, thereby ejaculating the ligand from the binding pocket, allowing it to activate the OR:CO-OR complex and the cellular signal transduction begins.
 *'''Activation by the complex pheromone-PBP'''