4u98: Difference between revisions

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'''Unreleased structure'''
==Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway (AppCp complex)==
<StructureSection load='4u98' size='340' side='right' caption='[[4u98]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4u98]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U98 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Maltokinase Maltokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.175 2.7.1.175] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u98 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u98 RCSB], [http://www.ebi.ac.uk/pdbsum/4u98 PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/MAK_MYCVP MAK_MYCVP]] Catalyzes the ATP-dependent phosphorylation of maltose to maltose 1-phosphate. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, GlgE and GlgB (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A novel four-step pathway identified recently in mycobacteria channels trehalose to glycogen synthesis and is also likely involved in the biosynthesis of two other crucial polymers: intracellular methylglucose lipopolysaccharides and exposed capsular glucan. The structures of three of the intervening enzymes - GlgB, GlgE, and TreS - were recently reported, providing the first templates for rational drug design. Here we describe the structural characterization of the fourth enzyme of the pathway, mycobacterial maltokinase (Mak), uncovering a eukaryotic-like kinase (ELK) fold, similar to methylthioribose kinases and aminoglycoside phosphotransferases. The 1.15 A structure of Mak in complex with a non-hydrolysable ATP analog reveals subtle structural rearrangements upon nucleotide binding in the cleft between the N- and the C-terminal lobes. Remarkably, this new family of ELKs has a novel N-terminal domain topologically resembling the cystatin family of protease inhibitors. By interfacing with and restraining the mobility of the phosphate-binding region of the N-terminal lobe, Mak's unusual N-terminal domain might regulate its phosphotransfer activity and represents the most likely anchoring point for TreS, the upstream enzyme in the pathway. By completing the gallery of atomic-detail models of an essential pathway, this structure opens new avenues for the rational design of alternative anti-tubercular compounds.


The entry 4u98 is ON HOLD  until Paper Publication
Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway.,Fraga J, Maranha A, Mendes V, Pereira PJ, Empadinhas N, Macedo-Ribeiro S Sci Rep. 2015 Jan 26;5:8026. doi: 10.1038/srep08026. PMID:25619172<ref>PMID:25619172</ref>


Authors: Fraga, J., Empadinhas, N., Pereira, P.J.B., Macedo-Ribeiro, S.
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
Description: Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway (AppCp complex)
== References ==
[[Category: Unreleased Structures]]
<references/>
__TOC__
</StructureSection>
[[Category: Maltokinase]]
[[Category: Empadinhas, N]]
[[Category: Empadinhas, N]]
[[Category: Fraga, J]]
[[Category: Macedo-Ribeiro, S]]
[[Category: Macedo-Ribeiro, S]]
[[Category: Pereira, P.J.B]]
[[Category: Pereira, P J.B]]
[[Category: Fraga, J]]
[[Category: Appcp]]
[[Category: Glycogen]]
[[Category: Maltose]]
[[Category: Transferase]]
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