4gyi: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[ | ==Crystal structure of the Rio2 kinase-ADP/Mg2+-phosphoaspartate complex from Chaetomium thermophilum== | ||
<StructureSection load='4gyi' size='340' side='right' caption='[[4gyi]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4gyi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_dsm_1495 Chaetomium thermophilum var. thermophilum dsm 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GYI FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gyg|4gyg]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0033330, Rio2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 Chaetomium thermophilum var. thermophilum DSM 1495])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gyi RCSB], [http://www.ebi.ac.uk/pdbsum/4gyi PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ribosome synthesis involves dynamic association of ribosome-biogenesis factors with evolving preribosomal particles. Rio2 is an atypical protein kinase required for pre-40S subunit maturation. We report the crystal structure of eukaryotic Rio2-ATP-Mg(2+) complex. The active site contains ADP-Mg(2+) and a phosphoaspartate intermediate typically found in Na(+), K(+) and Ca(2+) ATPases but not protein kinases. Consistent with this finding, ctRio2 exhibits a robust ATPase activity in vitro. In vivo, Rio2 docks on the ribosome, with its active site occluded and its flexible loop positioned to interact with the pre-40S subunit. Moreover, Rio2 catalytic activity is required for its dissociation from the ribosome, a necessary step in pre-40S maturation. We propose that phosphoryl transfer from ATP to Asp257 in Rio2's active site and subsequent hydrolysis of the aspartylphosphate could be a trigger to power late cytoplasmic 40S subunit biogenesis. | |||
ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit.,Ferreira-Cerca S, Sagar V, Schafer T, Diop M, Wesseling AM, Lu H, Chai E, Hurt E, Laronde-Leblanc N Nat Struct Mol Biol. 2012 Dec;19(12):1316-23. doi: 10.1038/nsmb.2403. Epub 2012, Oct 28. PMID:23104056<ref>PMID:23104056</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Chaetomium thermophilum var. thermophilum dsm 1495]] | [[Category: Chaetomium thermophilum var. thermophilum dsm 1495]] | ||
[[Category: Chai, E | [[Category: Chai, E]] | ||
[[Category: Diop, M | [[Category: Diop, M]] | ||
[[Category: Ferreira-Cerca, S | [[Category: Ferreira-Cerca, S]] | ||
[[Category: Hurt, E | [[Category: Hurt, E]] | ||
[[Category: LaRonde-LeBlanc, N | [[Category: LaRonde-LeBlanc, N]] | ||
[[Category: Lu, H | [[Category: Lu, H]] | ||
[[Category: Sagar, V | [[Category: Sagar, V]] | ||
[[Category: Schafer, T | [[Category: Schafer, T]] | ||
[[Category: Wesseling, A M | [[Category: Wesseling, A M]] | ||
[[Category: Acyl-phosphate]] | [[Category: Acyl-phosphate]] | ||
[[Category: Adp complex]] | [[Category: Adp complex]] | ||
