2puj: Difference between revisions

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[[Image:2puj.jpg|left|200px]]<br /><applet load="2puj" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:2puj.jpg|left|200px]]
caption="2puj, resolution 1.57&Aring;" />
 
'''Crystal Structure of the S112A/H265A double mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA'''<br />
{{Structure
|PDB= 2puj |SIZE=350|CAPTION= <scene name='initialview01'>2puj</scene>, resolution 1.57&Aring;
|SITE=
|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene> and <scene name='pdbligand=HPZ:(2E,4E)-2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID'>HPZ</scene>
|ACTIVITY=
|GENE= bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])
}}
 
'''Crystal Structure of the S112A/H265A double mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
2PUJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=MLI:'>MLI</scene> and <scene name='pdbligand=HPZ:'>HPZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUJ OCA].  
2PUJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUJ OCA].  


==Reference==
==Reference==
The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad., Horsman GP, Bhowmik S, Seah SY, Kumar P, Bolin JT, Eltis LD, J Biol Chem. 2007 Jul 6;282(27):19894-904. Epub 2007 Apr 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17442675 17442675]
The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad., Horsman GP, Bhowmik S, Seah SY, Kumar P, Bolin JT, Eltis LD, J Biol Chem. 2007 Jul 6;282(27):19894-904. Epub 2007 Apr 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17442675 17442675]
[[Category: Burkholderia xenovorans]]
[[Category: Burkholderia xenovorans]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: c-c bond hydrolase]]
[[Category: c-c bond hydrolase]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:33:06 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:17:32 2008''
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