2ycb: Difference between revisions
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==STRUCTURE OF THE ARCHAEAL BETA-CASP PROTEIN WITH N-TERMINAL KH DOMAINS FROM METHANOTHERMOBACTER THERMAUTOTROPHICUS== | |||
<StructureSection load='2ycb' size='340' side='right' caption='[[2ycb]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2ycb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YCB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YCB FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ycb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ycb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ycb RCSB], [http://www.ebi.ac.uk/pdbsum/2ycb PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
MTH1203, a beta-CASP metallo-beta-lactamase family nuclease from the archaeon Methanothermobacter thermautotrophicus, was identified as a putative nuclease that might contribute to RNA processing. The crystal structure of MTH1203 reveals that, in addition to the metallo-beta-lactamase nuclease and the beta-CASP domains, it contains two contiguous KH domains that are unique to MTH1203 and its orthologs. RNA-binding experiments indicate that MTH1203 preferentially binds U-rich sequences with a dissociation constant in the micromolar range. In vitro nuclease activity assays demonstrated that MTH1203 is a zinc-dependent nuclease. MTH1203 is also shown to be a dimer and, significantly, this dimerization enhances the nuclease activity. Transcription termination in archaea produces mRNA transcripts with U-rich 3' ends that could be degraded by MTH1203 considering its RNA-binding specificity. We hypothesize that this nuclease degrades mRNAs of proteins targeted for degradation and so regulates archaeal RNA turnover, possibly in concert with the exosome. | |||
Structure and Activity of a Novel Archaeal beta-CASP Protein with N-Terminal KH Domains.,Silva AP, Chechik M, Byrne RT, Waterman DG, Ng CL, Dodson EJ, Koonin EV, Antson AA, Smits C Structure. 2011 May 11;19(5):622-32. PMID:21565697<ref>PMID:21565697</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Methanothermobacter thermautotrophicus]] | [[Category: Methanothermobacter thermautotrophicus]] | ||
[[Category: Antson, A A | [[Category: Antson, A A]] | ||
[[Category: Byrne, R T | [[Category: Byrne, R T]] | ||
[[Category: Chechik, M | [[Category: Chechik, M]] | ||
[[Category: Dodson, E J | [[Category: Dodson, E J]] | ||
[[Category: Koonin, E V | [[Category: Koonin, E V]] | ||
[[Category: Ng, C L | [[Category: Ng, C L]] | ||
[[Category: Silva, A P.G | [[Category: Silva, A P.G]] | ||
[[Category: Smits, C | [[Category: Smits, C]] | ||
[[Category: Waterman, D G | [[Category: Waterman, D G]] | ||
[[Category: Beta-casp]] | [[Category: Beta-casp]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
