2pvi: Difference between revisions
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[[Image:2pvi.gif|left|200px]] | [[Image:2pvi.gif|left|200px]] | ||
'''PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA''' | {{Structure | ||
|PDB= 2pvi |SIZE=350|CAPTION= <scene name='initialview01'>2pvi</scene>, resolution 1.76Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] | |||
|GENE= | |||
}} | |||
'''PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2PVI is a [ | 2PVI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PVI OCA]. | ||
==Reference== | ==Reference== | ||
How is modification of the DNA substrate recognized by the PvuII restriction endonuclease?, Horton JR, Bonventre J, Cheng X, Biol Chem. 1998 Apr-May;379(4-5):451-8. PMID:[http:// | How is modification of the DNA substrate recognized by the PvuII restriction endonuclease?, Horton JR, Bonventre J, Cheng X, Biol Chem. 1998 Apr-May;379(4-5):451-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9628337 9628337] | ||
[[Category: Proteus vulgaris]] | [[Category: Proteus vulgaris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: complex (restriction endonuclease/dna)]] | [[Category: complex (restriction endonuclease/dna)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:17:57 2008'' | ||
Revision as of 16:17, 20 March 2008
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| 2pvi, resolution 1.76Å | |||||||||||||
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| Activity: | Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA
Overview
In restriction-modification systems, cleavage of substrate sites in cellular DNA by the restriction endonuclease is prevented by the action of a cognate methyltransferase that acts on the same substrate sites. The PvuII restriction endonuclease (R.PvuII) has been structurally characterized in a complex with substrate DNA (Cheng et al., 1994) and as an apoenzyme (Athanasiadis et al., 1994). We report here a structure, determined to 1.9 A resolution by crystallography, of a complex between R.PvuII and iodinated DNA. The presence of an iodine at the 5-carbon of the methylatable cytosine results in the following changes in the protein: His84 moved away from the modified base; this movement was amplified in His85 and disrupts an intersubunit hydrogen bond; and the base modification disturbs the distribution of water molecules that associate with these histidine residues and the area of the scissile bond. Considering these observations, hypotheses are given as to why a similar oligonucleotide, where a methyl group resides on the 5-carbon of the methylatable cytosine, is slowly cleaved by R.PvuII (Rice et al., 1995).
About this Structure
2PVI is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.
Reference
How is modification of the DNA substrate recognized by the PvuII restriction endonuclease?, Horton JR, Bonventre J, Cheng X, Biol Chem. 1998 Apr-May;379(4-5):451-8. PMID:9628337
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