1ema

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA

spinqualitylabelsall models 1ema, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1ema is a 1 chain structure with sequence from Aequorea victoria. The June 2003 RCSB PDB Molecule of the Month feature on Green Fluorescent Protein (GFP) by David S. Goodsell is 10.2210/rcsb_pdb/mom_2003_6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: CRO, MSE Resources: FirstGlance, OCA, RCSB, PDBsum Function [GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The chromophore, resulting from the spontaneous cyclization and oxidation of the sequence -Ser65 (or Thr65)-Tyr66-Gly67-, requires the native protein fold for both formation and fluorescence emission. The structure of Thr65 GFP has been determined at 1.9 angstrom resolution. The protein fold consists of an 11-stranded beta barrel with a coaxial helix, with the chromophore forming from the central helix. Directed mutagenesis of one residue adjacent to the chromophore, Thr203, to Tyr or His results in significantly red-shifted excitation and emission maxima. Crystal structure of the Aequorea victoria green fluorescent protein.,Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Gfp vc2 Green Fluorescent Protein Green Fluorescent Protein: Research Tool Proteopedia.org/wiki/index.php/User:Joel L. Sussman/GFP Test Page Proteopedia.org/wiki/workshop/GFP Background User:Joel L. Sussman/GFP Test Page User:Lynmarie K Thompson/2009Chem490 User:Shai Biran/GFP test page User:Shai Biran/Practice Proteopedia tutorial shai2 User:Shai Biran/Practice Proteopedia tutorial shai3 User:Shai Biran/Practice Proteopedia tutorial shai4 User:Shai Biran/Practice Proteopedia tutorial shai5

Proteopedia Page Contributors and Editors (what is this?)

OCA