2pzx: Difference between revisions
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[[Image:2pzx.jpg|left|200px]] | [[Image:2pzx.jpg|left|200px]] | ||
'''Structure of the methuselah ectodomain with peptide inhibitor''' | {{Structure | ||
|PDB= 2pzx |SIZE=350|CAPTION= <scene name='initialview01'>2pzx</scene>, resolution 3.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= mth ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | |||
}} | |||
'''Structure of the methuselah ectodomain with peptide inhibitor''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2PZX is a [ | 2PZX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZX OCA]. | ||
==Reference== | ==Reference== | ||
Extension of Drosophila melanogaster life span with a GPCR peptide inhibitor., Ja WW, West AP Jr, Delker SL, Bjorkman PJ, Benzer S, Roberts RW, Nat Chem Biol. 2007 Jul;3(7):415-9. Epub 2007 Jun 3. PMID:[http:// | Extension of Drosophila melanogaster life span with a GPCR peptide inhibitor., Ja WW, West AP Jr, Delker SL, Bjorkman PJ, Benzer S, Roberts RW, Nat Chem Biol. 2007 Jul;3(7):415-9. Epub 2007 Jun 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17546039 17546039] | ||
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: signaling protein]] | [[Category: signaling protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:20:00 2008'' | ||
Revision as of 16:20, 20 March 2008
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| 2pzx, resolution 3.50Å | |||||||||||||
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| Gene: | mth (Drosophila melanogaster) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of the methuselah ectodomain with peptide inhibitor
Overview
G protein-coupled receptors (GPCRs) mediate signaling from extracellular ligands to intracellular signal transduction proteins. Methuselah (Mth) is a class B (secretin-like) GPCR, a family typified by their large, ligand-binding, N-terminal extracellular domains. Downregulation of mth increases the life span of Drosophila melanogaster; inhibitors of Mth signaling should therefore enhance longevity. We used mRNA display selection to identify high-affinity (K(d) = 15 to 30 nM) peptide ligands that bind to the N-terminal ectodomain of Mth. The selected peptides are potent antagonists of Mth signaling, and structural studies suggest that they perturb the interface between the Mth ecto- and transmembrane domains. Flies constitutively expressing a Mth antagonist peptide have a robust life span extension, which suggests that the peptides inhibit Mth signaling in vivo. Our work thus provides new life span-extending ligands for a metazoan and a general approach for the design of modulators of this important class of GPCRs.
About this Structure
2PZX is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Extension of Drosophila melanogaster life span with a GPCR peptide inhibitor., Ja WW, West AP Jr, Delker SL, Bjorkman PJ, Benzer S, Roberts RW, Nat Chem Biol. 2007 Jul;3(7):415-9. Epub 2007 Jun 3. PMID:17546039
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