4rhf: Difference between revisions
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''' | ==Crystal structure of UbiX mutant V47S from Colwellia psychrerythraea 34H== | ||
<StructureSection load='4rhf' size='340' side='right' caption='[[4rhf]], [[Resolution|resolution]] 1.76Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4rhf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHF FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rhe|4rhe]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rhf RCSB], [http://www.ebi.ac.uk/pdbsum/4rhf PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The ubiX gene of Colwellia psychrerythraea strain 34H encodes a 3-octaprenyl-4-hydroxybenzoate carboxylase (CpsUbiX, UniProtKB code: Q489U8) that is involved in the third step of the ubiquinone biosynthesis pathway and harbors a flavin mononucleotide (FMN) as a potential cofactor. Here, we report the crystal structures of two forms of CpsUbiX: an FMN-bound wild type form and an FMN-unbound V47S mutant form. CpsUbiX is a dodecameric enzyme, and each monomer possesses a typical Rossmann-fold structure. The FMN-binding domain of UbiX is composed of three neighboring subunits. The highly conserved Gly15, Ser41, Val47, and Tyr171 residues play important roles in FMN binding. Structural comparison of the FMN-bound wild type form with the FMN-free form reveals a significant conformational difference in the C-terminal loop region (comprising residues 170-176 and 195-206). Subsequent computational modeling and liposome binding assay both suggest that the conformational flexibility observed in the C-terminal loops plays an important role in substrate and lipid bindings. The crystal structures presented in this work provide structural framework and insights into the catalytic mechanism of CpsUbiX. | |||
Crystal structure of UbiX, an aromatic acid decarboxylase from the psychrophilic bacterium Colwellia psychrerythraea that undergoes FMN-induced conformational changes.,Do H, Kim SJ, Lee CW, Kim HW, Park HH, Kim HM, Park H, Park H, Lee JH Sci Rep. 2015 Feb 3;5:8196. doi: 10.1038/srep08196. PMID:25645665<ref>PMID:25645665</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Do, H]] | [[Category: Do, H]] | ||
[[Category: Kim, H M]] | |||
[[Category: Kim, H W]] | |||
[[Category: Kim, S J]] | |||
[[Category: Lee, C W]] | |||
[[Category: Lee, J H]] | |||
[[Category: Park, H]] | [[Category: Park, H]] | ||
[[Category: | [[Category: Park, H H]] | ||
[[Category: | [[Category: Park, H J]] | ||
[[Category: | [[Category: Decarboxylation]] | ||
[[Category: Lyase]] | |||
[[Category: Rossmann fold]] | |||
Revision as of 12:59, 18 February 2015
Crystal structure of UbiX mutant V47S from Colwellia psychrerythraea 34H
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