2bf0: Difference between revisions

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==Overview==

The C-terminal domain (CTD) of the large subunit of RNA polymerase II is a, platform for mRNA processing factors and links gene transcription to mRNA, capping, splicing and polyadenylation. Pcf11, an essential component of, the mRNA cleavage factor IA, contains a CTD-interaction domain that binds, in a phospho-dependent manner to the heptad repeats within the RNA, polymerase II CTD. We show here that the phosphorylated CTD exists as a, dynamic disordered ensemble in solution and, by induced fit, it assumes a, structured conformation when bound to Pcf11. In addition, we detected, cis-trans populations for the CTD prolines, and found that only the, all-trans form is selected for binding. These data suggest that the, recognition of the CTD is regulated by independent site-specific, ~~... [[http://ispc.weizmann.ac.il/pmbin/getpm?15665873~~ (~~full description~~)]]

The C-terminal domain (CTD) of the large subunit of RNA polymerase II is a, platform for mRNA processing factors and links gene transcription to mRNA, capping, splicing and polyadenylation. Pcf11, an essential component of, the mRNA cleavage factor IA, contains a CTD-interaction domain that binds, in a phospho-dependent manner to the heptad repeats within the RNA, polymerase II CTD. We show here that the phosphorylated CTD exists as a, dynamic disordered ensemble in solution and, by induced fit, it assumes a, structured conformation when bound to Pcf11. In addition, we detected, cis-trans populations for the CTD prolines, and found that only the, all-trans form is selected for binding. These data suggest that the, recognition of the CTD is regulated by independent site-specific, modifications (phosphorylation and proline cis-trans isomerization) and, probably, by the local concentration of suitable binding sites.

==About this Structure==

2BF0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: CAL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BF0 OCA]].

2BF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: CAL. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BF0 OCA].

==Reference==

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[[Category: transcription]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on ~~Tue Oct 30 16~~:35:26 2007''

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:12:34 2007''

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@@ Line 5: / Line 5: @@
 ==Overview==
-The C-terminal domain (CTD) of the large subunit of RNA polymerase II is a, platform for mRNA processing factors and links gene transcription to mRNA, capping, splicing and polyadenylation. Pcf11, an essential component of, the mRNA cleavage factor IA, contains a CTD-interaction domain that binds, in a phospho-dependent manner to the heptad repeats within the RNA, polymerase II CTD. We show here that the phosphorylated CTD exists as a, dynamic disordered ensemble in solution and, by induced fit, it assumes a, structured conformation when bound to Pcf11. In addition, we detected, cis-trans populations for the CTD prolines, and found that only the, all-trans form is selected for binding. These data suggest that the, recognition of the CTD is regulated by independent site-specific, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15665873 (full description)]]
+The C-terminal domain (CTD) of the large subunit of RNA polymerase II is a, platform for mRNA processing factors and links gene transcription to mRNA, capping, splicing and polyadenylation. Pcf11, an essential component of, the mRNA cleavage factor IA, contains a CTD-interaction domain that binds, in a phospho-dependent manner to the heptad repeats within the RNA, polymerase II CTD. We show here that the phosphorylated CTD exists as a, dynamic disordered ensemble in solution and, by induced fit, it assumes a, structured conformation when bound to Pcf11. In addition, we detected, cis-trans populations for the CTD prolines, and found that only the, all-trans form is selected for binding. These data suggest that the, recognition of the CTD is regulated by independent site-specific, modifications (phosphorylation and proline cis-trans isomerization) and, probably, by the local concentration of suitable binding sites.
 ==About this Structure==
-BF0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: CAL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BF0 OCA]].
+BF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: CAL. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BF0 OCA].
 ==Reference==
@@ Line 27: / Line 27: @@
 [[Category: transcription]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:35:26 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:12:34 2007''