Sandbox Reserved 994: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Josie Werner (talk | contribs) No edit summary |
Josie Werner (talk | contribs) No edit summary |
||
| Line 26: | Line 26: | ||
<scene name='69/691536/Closeupdrug/1'>close up</scene><ref>PMID: 10817708</ref> | <scene name='69/691536/Closeupdrug/1'>close up</scene><ref>PMID: 10817708</ref> | ||
== Catalytic Activity == | |||
There are three catalytic residues involved in the hydrolysis of β-lactam antibiotics. Serine-81 is the catalytic serine, which performs a nucleophilic attack on the β-lactam ring after being deprotonated by the carboxylated lysine-84 (KCX84). This carboxylated lysine is formed by CO2 in the environment engaging in unfavorable interactions with the hydrophobic pocket of OXA-24, so it carboxylates Lysine-84 (A).<ref name="Leonard" /> | There are three catalytic residues involved in the hydrolysis of β-lactam antibiotics. Serine-81 is the catalytic serine, which performs a nucleophilic attack on the β-lactam ring after being deprotonated by the carboxylated lysine-84 (KCX84). This carboxylated lysine is formed by CO2 in the environment engaging in unfavorable interactions with the hydrophobic pocket of OXA-24, so it carboxylates Lysine-84 (A).<ref name="Leonard" /> | ||
