4xc2: Difference between revisions

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'''Unreleased structure'''
==Crystal structure of GABARAP in complex with KBTBD6 LIR peptide==
<StructureSection load='4xc2' size='340' side='right' caption='[[4xc2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xc2]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XC2 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xc2 RCSB], [http://www.ebi.ac.uk/pdbsum/4xc2 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The small Rho GTPase RAC1 is an essential regulator of cellular signaling that controls actin rearrangements and cell motility. Here, we identify a novel CUL3 RING ubiquitin ligase complex, containing the substrate adaptors KBTBD6 and KBTBD7, that mediates ubiquitylation and proteasomal degradation of TIAM1, a RAC1-specific GEF. Increasing the abundance of TIAM1 by depletion of KBTBD6 and/or KBTBD7 leads to elevated RAC1 activity, changes in actin morphology, loss of focal adhesions, reduced proliferation, and enhanced invasion. KBTBD6 and KBTBD7 employ ATG8 family-interacting motifs to bind preferentially to GABARAP proteins. Surprisingly, ubiquitylation and degradation of TIAM1 by CUL3KBTBD6/KBTBD7 depends on its binding to GABARAP proteins. Our study reveals that recruitment of CUL3KBTBD6/KBTBD7 to GABARAP-containing vesicles regulates the abundance of membrane-associated TIAM1 and subsequently spatially restricted RAC1 signaling. Besides their role in autophagy and trafficking, we uncovered a previously unknown function of GABARAP proteins as membrane-localized signaling scaffolds.


The entry 4xc2 is ON HOLD  until Paper Publication
CUL3-KBTBD6/KBTBD7 Ubiquitin Ligase Cooperates with GABARAP Proteins to Spatially Restrict TIAM1-RAC1 Signaling.,Genau HM, Huber J, Baschieri F, Akutsu M, Dotsch V, Farhan H, Rogov V, Behrends C Mol Cell. 2015 Feb 11. pii: S1097-2765(14)01018-1. doi:, 10.1016/j.molcel.2014.12.040. PMID:25684205<ref>PMID:25684205</ref>


Authors: Huber, J., Genau, H.M., Baschieri, F., Doetsch, V., Farhan, H., Rogov, V., Behrends, C., Akutsu, M.
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
Description: Crystal structure of GABARAP in complex with KBTBD6 LIR peptide
== References ==
[[Category: Unreleased Structures]]
<references/>
__TOC__
</StructureSection>
[[Category: Akutsu, M]]
[[Category: Akutsu, M]]
[[Category: Baschieri, F]]
[[Category: Behrends, C]]
[[Category: Doetsch, V]]
[[Category: Doetsch, V]]
[[Category: Farhan, H]]
[[Category: Genau, H M]]
[[Category: Huber, J]]
[[Category: Huber, J]]
[[Category: Baschieri, F]]
[[Category: Farhan, H]]
[[Category: Rogov, V]]
[[Category: Rogov, V]]
[[Category: Behrends, C]]
[[Category: Autophagy]]
[[Category: Genau, H.M]]
[[Category: Complex]]
[[Category: Immune system]]
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