2r2i: Difference between revisions
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[[Image:2r2i.jpg|left|200px]] | [[Image:2r2i.jpg|left|200px]] | ||
'''Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound''' | {{Structure | ||
|PDB= 2r2i |SIZE=350|CAPTION= <scene name='initialview01'>2r2i</scene>, resolution 2.000Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene> and <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene> | |||
|ACTIVITY= | |||
|GENE= GUCA1A, GCAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | |||
}} | |||
'''Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2R2I is a [ | 2R2I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2I OCA]. | ||
==Reference== | ==Reference== | ||
Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1., Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC, Structure. 2007 Nov;15(11):1392-402. PMID:[http:// | Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1., Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC, Structure. 2007 Nov;15(11):1392-402. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17997965 17997965] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ef hand]] | [[Category: ef hand]] | ||
[[Category: gcap]] | [[Category: gcap]] | ||
[[Category: gcap-1 ]] | [[Category: gcap-1]] | ||
[[Category: gcap1]] | [[Category: gcap1]] | ||
[[Category: guanylate cyclase activating protein]] | [[Category: guanylate cyclase activating protein]] | ||
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[[Category: vision]] | [[Category: vision]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:31:55 2008'' | ||
Revision as of 16:31, 20 March 2008
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| 2r2i, resolution 2.000Å | |||||||||||||
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| Ligands: | CA, MYR and BME | ||||||||||||
| Gene: | GUCA1A, GCAP1 (Gallus gallus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound
Overview
Guanylate cyclase-activating proteins (GCAPs) are Ca(2+)-binding proteins myristoylated at the N terminus that regulate guanylate cyclases in photoreceptor cells and belong to the family of neuronal calcium sensors (NCS). Many NCS proteins display a recoverin-like "calcium-myristoyl switch" whereby the myristoyl group, buried inside the protein in the Ca(2+)-free state, becomes fully exposed upon Ca(2+) binding. Here we present a 2.0 A resolution crystal structure of myristoylated GCAP1 with Ca(2+) bound. The acyl group is buried inside Ca(2+)-bound GCAP1. This is in sharp contrast to Ca(2+)-bound recoverin, where the myristoyl group is solvent exposed. Furthermore, we provide direct evidence that the acyl group in GCAP1 remains buried in the Ca(2+)-free state and does not undergo switching. A pronounced kink in the C-terminal helix and the presence of the myristoyl group allow clustering of sequence elements crucial for GCAP1 activity.
About this Structure
2R2I is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1., Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC, Structure. 2007 Nov;15(11):1392-402. PMID:17997965
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