Sandbox Reserved 993: Difference between revisions
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Below is a schematic representation of the ''Photinus pyralis'' lucerifase reaction.<ref name=Branchini1998 /> | Below is a schematic representation of the ''Photinus pyralis'' lucerifase reaction.<ref name=Branchini1998 /> | ||
Step 1: ''Photinus pyralis'' luciferase catalyzes the production of luciferyl-AMP from luciferin and ATP. An inorganic pyrophosphate is released in this reaction. | Step 1: ''Photinus pyralis'' luciferase catalyzes the production of luciferyl-AMP from luciferin and ATP. An inorganic pyrophosphate is released in this reaction. | ||
Step 2: ''Photinus pyralis'' luciferase then converts luciferyl-AMP and O<sub>2</sub> into oxyluciferin* and CO<sub>2</sub>.<ref name=White1980 /><ref name=Thorne2012>Thorne, N., Shen, M., Lea, W. A., Simeonov, A., Lovell, S., Auld, D. S. and Inglese, J. (2012) "Firefly luciferase in chemical biology: A compendium of inhibitor, mechanistic evaluation of chemotypes, and suggested use as a reporter", Chem. Biol. 19(8): 1060-1072. doi:http://dx.doi.org/10.1016%2Fj.chembiol.2012.07.015</ref> When the electrons | |||
Step 2: ''Photinus pyralis'' luciferase then converts luciferyl-AMP and O<sub>2</sub> into oxyluciferin* and CO<sub>2</sub>.<ref name=White1980 /><ref name=Thorne2012>Thorne, N., Shen, M., Lea, W. A., Simeonov, A., Lovell, S., Auld, D. S. and Inglese, J. (2012) "Firefly luciferase in chemical biology: A compendium of inhibitor, mechanistic evaluation of chemotypes, and suggested use as a reporter", Chem. Biol. 19(8): 1060-1072. doi:http://dx.doi.org/10.1016%2Fj.chembiol.2012.07.015</ref> When the excited electrons in oxyluciferin* relax, light is emitted. | |||
[[Image:Luciferin_Mechanism.jpg]] | [[Image:Luciferin_Mechanism.jpg]] | ||
