Practical Guide to Homology Modeling: Difference between revisions

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Eric Martz (talk | contribs)
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*Click ''Amino Acids''.
*Click ''Amino Acids''.
*Click YES there is a known protein structure.
*Click YES there is a known protein structure.
*Click ''Choose File'' to upload your homology model. Click ''Next''.
*Enter your PDB code, or click ''Choose File'' to upload a homology model. Click ''Next''.
*Select chain A.
*Select the chain of interest. For a homology model, there will usually be only one chain, "A".
*Select NO you have not prepared a Multiple Sequence Alignment (MSA) that you wish to upload. The server will generate the MSA for you.
*Select NO you have not prepared a Multiple Sequence Alignment (MSA) that you wish to upload. The server will generate the MSA for you.
*Change ''number of iterations'' to 1.
*Check 'Let me select the sequences'. Leave all other settings at their defaults.
*Check 'Let me select the sequences'. Leave all other settings at their defaults.
*Enter a job title and your email address, then click the ''Submit'' button. The first step, gathering similar sequences, typically takes less than 5 minutes.
*Enter a job title and your email address, then click the ''Submit'' button. The first step, gathering similar sequences, typically takes less than 5 minutes.