Sandbox Reserved 987: Difference between revisions
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Cocaine esterase works in a four step mechanism.<sup><ref>Liu J., Zhao X., Yang W., Zhan G. C. (2011) Reaction mechanism for cocaine esterase-catalyzed hydrolysis of (+)- and (-)-cocaine: unexpected common rate-determining step. J. Phys. Chem. B. 115(17), 5017-5025.</ref></sup> | Cocaine esterase works in a four step mechanism.<sup><ref>Liu J., Zhao X., Yang W., Zhan G. C. (2011) Reaction mechanism for cocaine esterase-catalyzed hydrolysis of (+)- and (-)-cocaine: unexpected common rate-determining step. J. Phys. Chem. B. 115(17), 5017-5025.</ref></sup> | ||
1. The lone pair on from Ser117 attacks the ester. | 1. The lone pair on from Ser117 attacks the ester. | ||
2. The extra pair of electrons on the oxygen reform the double bond and ecgonine methyl ester leaves. | 2. The extra pair of electrons on the oxygen reform the double bond and ecgonine methyl ester leaves. | ||
3. A water molecules, stabilized by His287, attacks the ester again. | 3. A water molecules, stabilized by His287, attacks the ester again. | ||
4. The extra pair of electrons reform the double bond again and this time break the bond to Ser117, regenerating the catalyst. | 4. The extra pair of electrons reform the double bond again and this time break the bond to Ser117, regenerating the catalyst. | ||
