Sandbox Reserved 1066: Difference between revisions
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[[Image:acyl coa synthetase.jpg|425 px|right|thumb|Figure 2: Representation of the two-step reaction catalyzed by FadD13]] | [[Image:acyl coa synthetase.jpg|425 px|right|thumb|Figure 2: Representation of the two-step reaction catalyzed by FadD13]] | ||
== General mechanism for the activation of fatty acids == | == General mechanism for the activation of fatty acids == | ||
FadD13 represents the first Fatty Acyl-CoA Synthetase of its kind to display biphasic kinetics.<ref name="residue paper"/> FadD13 first activates the fatty acid through a reaction with ATP to form an acyl adenylate intermediate and subsequently releases a pyrophosphate. Following a conformational change of the enzyme upon the binding of ATP, coenzyme A is able to bind to its active site and react with the acyl adenylate intermediate forming the [http://en.wikipedia.org/wiki/Acyl-CoA acyl CoA] product (Figure 2). These activated fatty acyl-CoA thioesters have then been demonstrated to be important for the synthesis of triacyglycerols and phospholipids in the membrane of ''Mycobacterium tuberculosis''. <ref name="residue paper"/> | FadD13 represents the first Fatty Acyl-CoA Synthetase of its kind to display biphasic kinetics.<ref name="residue paper"/> FadD13 first activates the fatty acid through a reaction with ATP to form an acyl adenylate intermediate and subsequently releases a pyrophosphate. Following a conformational change of the enzyme upon the binding of ATP, coenzyme A is able to bind to its active site and react with the acyl adenylate intermediate forming the [http://en.wikipedia.org/wiki/Acyl-CoA acyl CoA] product (Figure 2). These activated fatty acyl-CoA thioesters have then been demonstrated to be important for the synthesis of triacyglycerols and phospholipids in the membrane of ''Mycobacterium tuberculosis''. <ref name="residue paper"/> | ||
