1ofu: Difference between revisions
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==Overview== | ==Overview== | ||
SulA halts cell division in Escherichia coli by binding to the major, component of the division machinery FtsZ. We have solved the crystal, structure of SulA alone and in complex with FtsZ from Pseudomonas, aeruginosa. SulA is expressed when the SOS response is induced. This is a, mechanism to inhibit cell division and repair DNA in the event of DNA, damage. FtsZ is a tubulin-like protein that forms polymers, with the, active-site GTPase split across two monomers. One monomer provides the, GTP-binding site and the other, through its T7 loop nucleotide hydrolysis., Our structures show that SulA is a dimer, and that SulA inhibits cell, division neither by binding the nucleotide-binding site nor by inducing, conformational changes in FtsZ. Instead, SulA binds the T7 loop surface of, FtsZ, .. | SulA halts cell division in Escherichia coli by binding to the major, component of the division machinery FtsZ. We have solved the crystal, structure of SulA alone and in complex with FtsZ from Pseudomonas, aeruginosa. SulA is expressed when the SOS response is induced. This is a, mechanism to inhibit cell division and repair DNA in the event of DNA, damage. FtsZ is a tubulin-like protein that forms polymers, with the, active-site GTPase split across two monomers. One monomer provides the, GTP-binding site and the other, through its T7 loop nucleotide hydrolysis., Our structures show that SulA is a dimer, and that SulA inhibits cell, division neither by binding the nucleotide-binding site nor by inducing, conformational changes in FtsZ. Instead, SulA binds the T7 loop surface of, FtsZ, opposite the nucleotide-binding site, blocking polymer formation., These findings explain why GTP hydrolysis and polymer turnover are, required for SulA inhibition. | ||
==About this Structure== | ==About this Structure== | ||
1OFU is a | 1OFU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with GDP as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OFU OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: sula protein]] | [[Category: sula protein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:18:40 2007'' | ||
