2v9v: Difference between revisions
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[[Image:2v9v.jpg|left|200px]] | [[Image:2v9v.jpg|left|200px]] | ||
'''CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)''' | {{Structure | ||
|PDB= 2v9v |SIZE=350|CAPTION= <scene name='initialview01'>2v9v</scene>, resolution 1.10Å | |||
|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Chain+A'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2V9V is a [ | 2V9V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9V OCA]. | ||
==Reference== | ==Reference== | ||
Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB., Ganichkin O, Wahl MC, Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:[http:// | Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB., Ganichkin O, Wahl MC, Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17881825 17881825] | ||
[[Category: Moorella thermoacetica]] | [[Category: Moorella thermoacetica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein biosynthesis]] | [[Category: protein biosynthesis]] | ||
[[Category: protein conformational change]] | [[Category: protein conformational change]] | ||
[[Category: protein | [[Category: protein dynamic]] | ||
[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
[[Category: selenoprotein biosynthesis]] | [[Category: selenoprotein biosynthesis]] | ||
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[[Category: winged-helix domain]] | [[Category: winged-helix domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:44:44 2008'' | ||
Revision as of 16:44, 20 March 2008
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| 2v9v, resolution 1.10Å | |||||||||||||
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| Sites: | AC1 | ||||||||||||
| Ligands: | CL and NA | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)
Overview
The crystal structure of the first two winged-helix motifs of translation elongation factor SelB from Moorella thermoacetica has been determined at 1.1 A resolution. Compared with the previous structure of the two domains in conjunction with winged-helix modules 3 and 4, the first winged-helix domain underwent a substantial conformational change during which the alpha-helical and beta-sheet portions of the element opened up like a shell. This conformational rearrangement was elicited by a change in the orientation of Trp396, leading to the disclosure of a bona fide ligand-binding site in the direct vicinity of Trp396. Additionally, the C-terminal tail of the second domain followed a different path compared with the previous structure. It is conceivable that these conformational switches constitute part of the molecular mechanism that underlies the communication between the N-terminal part of SelB, which binds Sec-tRNA(Sec) and GTP, and the C-terminal part of the protein, which binds selenocysteine-insertion sequences.
About this Structure
2V9V is a Single protein structure of sequence from Moorella thermoacetica. Full crystallographic information is available from OCA.
Reference
Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB., Ganichkin O, Wahl MC, Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:17881825
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