Sandbox Reserved 1052: Difference between revisions
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== '''Structure''' == | == '''Structure''' == | ||
Ag85 C is a dimer of identical subunits (Figure 1), and quite a bit about the amino acid sequence and overall structure of Ag85C is known. The enzyme contains a [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad] comprised of [http://en.wikipedia.org/wiki/Serine serine], [http://en.wikipedia.org/wiki/Glutamic_acid glutamate], and [http://en.wikipedia.org/wiki/Histidine histidine]. | Ag85 C is a <scene name='69/694219/Dimer/1'>dimer</scene> of identical subunits (Figure 1), and quite a bit about the amino acid sequence and overall structure of Ag85C is known. The enzyme contains a [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad] comprised of [http://en.wikipedia.org/wiki/Serine serine], [http://en.wikipedia.org/wiki/Glutamic_acid glutamate], and [http://en.wikipedia.org/wiki/Histidine histidine]. | ||
This would lead us to think that we could inhibit mycolyl transfer and shut down the enzyme by directly inhibiting the serine residue of the <scene name='69/694218/Catalytic_triad/2'>catalytic triad</scene>, but we can instead modify a near by [http://en.wikipedia.org/wiki/Cysteine cysteine] residue and have the same effect. This was determined by a series of experiments in which scientists generated various mutants and modifications of the Ag85C enzyme.<ref> Research Collaboratory for Structural Bioinformatics Protein Database (RCSB PDB) Diacylglycerol acyltransferase/mycolyltransferase Ag85C - P9WQN9 (A85C_MYCTU). [Internet] San Diego (CA): University of San Diego; 2014 [cited 2015 March 16]. [http://dx.doi.org/10.2210/pdbp9wqn9/pdb DOI:10.2210/pdbp9wqn9/pdb]</ref> | This would lead us to think that we could inhibit mycolyl transfer and shut down the enzyme by directly inhibiting the serine residue of the <scene name='69/694218/Catalytic_triad/2'>catalytic triad</scene>, but we can instead modify a near by [http://en.wikipedia.org/wiki/Cysteine cysteine] residue and have the same effect. This was determined by a series of experiments in which scientists generated various mutants and modifications of the Ag85C enzyme.<ref> Research Collaboratory for Structural Bioinformatics Protein Database (RCSB PDB) Diacylglycerol acyltransferase/mycolyltransferase Ag85C - P9WQN9 (A85C_MYCTU). [Internet] San Diego (CA): University of San Diego; 2014 [cited 2015 March 16]. [http://dx.doi.org/10.2210/pdbp9wqn9/pdb DOI:10.2210/pdbp9wqn9/pdb]</ref> | ||
