2vhf: Difference between revisions
New page: left|200px<br /><applet load="2vhf" size="350" color="white" frame="true" align="right" spinBox="true" caption="2vhf, resolution 2.800Å" /> '''STRUCTURE OF THE CY... |
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[[Image:2vhf.jpg|left|200px]] | [[Image:2vhf.jpg|left|200px]] | ||
'''STRUCTURE OF THE CYLD USP DOMAIN''' | {{Structure | ||
|PDB= 2vhf |SIZE=350|CAPTION= <scene name='initialview01'>2vhf</scene>, resolution 2.800Å | |||
|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+B'>AC3</scene> and <scene name='pdbsite=AC4:Zn+Binding+Site+For+Chain+B'>AC4</scene> | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF THE CYLD USP DOMAIN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2VHF is a [ | 2VHF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VHF OCA]. | ||
==Reference== | ==Reference== | ||
The Structure of the CYLD USP Domain Explains Its Specificity for Lys63-Linked Polyubiquitin and Reveals a B Box Module., Komander D, Lord CJ, Scheel H, Swift S, Hofmann K, Ashworth A, Barford D, Mol Cell. 2008 Feb 29;29(4):451-64. PMID:[http:// | The Structure of the CYLD USP Domain Explains Its Specificity for Lys63-Linked Polyubiquitin and Reveals a B Box Module., Komander D, Lord CJ, Scheel H, Swift S, Hofmann K, Ashworth A, Barford D, Mol Cell. 2008 Feb 29;29(4):451-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18313383 18313383] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zn-binding domain]] | [[Category: zn-binding domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:46:38 2008'' | ||
Revision as of 16:46, 20 March 2008
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| 2vhf, resolution 2.800Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sites: | AC1, AC2, AC3 and AC4 | ||||||||||||
| Ligands: | ZN | ||||||||||||
| Activity: | Ubiquitin thiolesterase, with EC number 3.1.2.15 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF THE CYLD USP DOMAIN
Overview
The tumor suppressor CYLD antagonizes NF-kappaB and JNK signaling by disassembly of Lys63-linked ubiquitin chains synthesized in response to cytokine stimulation. Here we describe the crystal structure of the CYLD USP domain, revealing a distinctive architecture that provides molecular insights into its specificity toward Lys63-linked polyubiquitin. We identify regions of the USP domain responsible for this specificity and demonstrate endodeubiquitinase activity toward such chains. Pathogenic truncations of the CYLD C terminus, associated with the hypertrophic skin tumor cylindromatosis, disrupt the USP domain, accounting for loss of CYLD catalytic activity. A small zinc-binding B box domain, similar in structure to other crossbrace Zn-binding folds-including the RING domain found in E3 ubiquitin ligases-is inserted within the globular core of the USP domain. Biochemical and functional characterization of the B box suggests a role as a protein-interaction module that contributes to determining the subcellular localization of CYLD.
About this Structure
2VHF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The Structure of the CYLD USP Domain Explains Its Specificity for Lys63-Linked Polyubiquitin and Reveals a B Box Module., Komander D, Lord CJ, Scheel H, Swift S, Hofmann K, Ashworth A, Barford D, Mol Cell. 2008 Feb 29;29(4):451-64. PMID:18313383
Page seeded by OCA on Thu Mar 20 18:46:38 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Homo sapiens
- Single protein
- Ubiquitin thiolesterase
- Ashworth, A.
- Barford, D.
- Hofmann, K.
- Komander, D.
- Lord, C J.
- Scheel, H.
- Swift, S.
- ZN
- Alternative splicing
- Anti-oncogene
- B-box
- Cell cycle
- Cell signalling
- Cross-brace
- Cytokine signalling
- Cytoplasm
- Deubiquitinating enzyme
- Hydrolase
- Linkage specificity
- Lys63- linked
- Nf-kb
- Phosphorylation
- Protease
- Thiol protease
- Ubiquitin
- Ubl conjugation pathway
- Usp domain
- Zn-binding domain
