Sandbox Reserved 1066: Difference between revisions

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==='''Salicylate synthase (SS)'''===
'''Salicylate synthase (SS)'''
Chromate is converted to salicylate synthase by MbtI through an intermediate isochromate, bound to an enzyme . During this reaction, a pyruvate molecule is expelled after the intermediate step (Figure 9). Through inhibition studies, mimics of isochromate were made and bound with MbtI.  However, the results did not yield a clear modality  for isochromate biding to the active site.  Between the two different modalities, two amino acids remained involved in the binding: Arg405 and Tyr385.  This illudes to a very flexible binding site<ref name="2a">PMID:23108268</ref>.  
Chromate is converted to salicylate synthase and pyruvate by MbtI through an intermediate isochromate. The pyruvate molecule is expelled after the intermediate step and salicylate is incorporated in the biosynthesis of mycobactin T (Figure 9,10)<ref name="2a">PMID:23108268</ref>. Inhibition studies revealed two binding modes of MbtI based on the structure of the substrate <ref name="5a"/>. Mimics of isochromate inhibitors with modified enolpyruvly side chains showed the greatest inhibition capability and reoriented the substrate within the active side of the enzyme causing the backbone of the enzyme to shift away from the closed conformation (Figure 3,4,5)<ref name="5a"/>. A clear mechanism for the salicylate synthase activity of MbtI is currently unknown<ref name="5a"/>.  
 
[[Image:Image:Salicylate synthase chem draw.png]]


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