User:Wayne Decatur/Gal 4: Difference between revisions
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==DNA Recognition by Gal4p== | ==DNA Recognition by Gal4p== | ||
The protein binds as a dimer to a symmetrical 17-base-pair sequence. Specifically, the consensus Gal4p-binding site is a 17-mer of sequence conforming to the motif below.<br> | The protein <scene name='70/701975/1d66_firstglance/1'>binds as a dimer to a symmetrical 17-base-pair sequence</scene>. Specifically, the consensus Gal4p-binding site is a 17-mer of sequence conforming to the motif below.<br> | ||
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<span style="font-weight: bold;font-family: Courier New; font-size: 14pt"> | <span style="font-weight: bold;font-family: Courier New; font-size: 14pt"> | ||
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The DNA binding domain uses four Cys residues to coordinate the binding of two ions of zinc (Zn(II)). In the crystal structure [[1d66]], the zinc ions are represented by other members of the same group on the periodic table, the heavier cadmium ions. The Zn-binding motif lets the structure accomplish more with fewer amino acids, the metals lending an "economy of structure", arranging two helices to recognize a specific DNA site. | The DNA binding domain uses four Cys residues to coordinate the binding of two ions of zinc (Zn(II)). In the crystal structure [[1d66]], the zinc ions are represented by other members of the same group on the periodic table, the heavier cadmium ions. The Zn-binding motif lets the structure accomplish more with fewer amino acids, the metals lending an "economy of structure", arranging two small helices to recognize a specific DNA site. | ||
The third large helix of each dimer subunit interacts with the other <scene name='70/701975/1d66_firstglance_slab/1'>by arranging hydrophobic residues in the contact region</scene>. Note the abundance of gray residues in the central stem protruding from a site roughly the center of the DNA binding site. The dimer interface is more fully explored in a later publication with an expanded structure, see [[3coq]]. | |||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID:1557122</ref><references group="xtra"/> | <ref group="xtra">PMID:1557122</ref><references group="xtra"/> | ||
