2zfd: Difference between revisions
New page: left|200px<br /><applet load="2zfd" size="350" color="white" frame="true" align="right" spinBox="true" caption="2zfd, resolution 1.20Å" /> '''The crystal structur... |
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'''The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14''' | {{Structure | ||
|PDB= 2zfd |SIZE=350|CAPTION= <scene name='initialview01'>2zfd</scene>, resolution 1.20Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene> | |||
|ACTIVITY= | |||
|GENE= CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | |||
}} | |||
'''The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2ZFD is a [ | 2ZFD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. | ||
==Reference== | ==Reference== | ||
The Crystal Structure of Plant-Specific Calcium-Binding Protein AtCBL2 in Complex with the Regulatory Domain of AtCIPK14., Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T, J Mol Biol. 2008 Jan 11;. PMID:[http:// | The Crystal Structure of Plant-Specific Calcium-Binding Protein AtCBL2 in Complex with the Regulatory Domain of AtCIPK14., Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T, J Mol Biol. 2008 Jan 11;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18237745 18237745] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:54:06 2008'' | ||
Revision as of 16:54, 20 March 2008
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| 2zfd, resolution 1.20Å | |||||||||||||
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| Ligands: | CA and ACY | ||||||||||||
| Gene: | CBL2 (Arabidopsis thaliana), CIPK14 (Arabidopsis thaliana) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14
Overview
Calcium signals mediate a multitude of plant responses to external stimuli. Calcineurin B-like (CBL) proteins and their target kinases, CBL-interacting protein kinases (CIPKs), represent important relays in plant calcium signaling. CBL interacts with CIPK through a conserved motif (NAF/FISL motif) within the C-terminal regulatory domain. To better understand the functional role of the CBL-CIPK system, we determined the crystal structure of AtCBL2 in complex with the regulatory domain of AtCIPK14 at 1.2 A resolution. The NAF/FISL motif is inserted into a hydrophobic crevice within AtCBL2, accompanied by a large displacement of the helices and loop on the opposite side of the NAF/FISL motif from the C-terminal region, which shields the hydrophobic crevice in free form. Ca(2+) are coordinated within four EF hands in AtCBL2 in bound form. This calcium coordination pattern differs from that in the structure of the SOS3-SOS2 complex previously reported. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity.
About this Structure
2ZFD is a Protein complex structure of sequences from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
The Crystal Structure of Plant-Specific Calcium-Binding Protein AtCBL2 in Complex with the Regulatory Domain of AtCIPK14., Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T, J Mol Biol. 2008 Jan 11;. PMID:18237745
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