378d: Difference between revisions
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[[Image:378d.gif|left|200px]] | [[Image:378d.gif|left|200px]] | ||
'''STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA''' | {{Structure | ||
|PDB= 378d |SIZE=350|CAPTION= <scene name='initialview01'>378d</scene>, resolution 2.400Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
378D is a [ | 378D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http:// | Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089456 10089456] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Mitra, S N.]] | [[Category: Mitra, S N.]] | ||
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[[Category: right handed dna]] | [[Category: right handed dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:06 2008'' | ||
Revision as of 16:55, 20 March 2008
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| 378d, resolution 2.400Å | |||||||||||||
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| Ligands: | DMY and NA | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA
Overview
The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.
About this Structure
378D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:10089456
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