3bdq: Difference between revisions
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[[Image:3bdq.jpg|left|200px]] | [[Image:3bdq.jpg|left|200px]] | ||
'''Room Tempreture Crystal Structure of Sterol Carrier Protein-2 Like-2''' | {{Structure | ||
|PDB= 3bdq |SIZE=350|CAPTION= <scene name='initialview01'>3bdq</scene>, resolution 2.000Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PLM:PALMITIC ACID'>PLM</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Room Tempreture Crystal Structure of Sterol Carrier Protein-2 Like-2''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
3BDQ is a [ | 3BDQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDQ OCA]. | ||
==Reference== | ==Reference== | ||
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:[http:// | Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18084051 18084051] | ||
[[Category: Aedes aegypti]] | [[Category: Aedes aegypti]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sterol carrier protein]] | [[Category: sterol carrier protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:58:10 2008'' | ||
Revision as of 16:58, 20 March 2008
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| 3bdq, resolution 2.000Å | |||||||||||||
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| Ligands: | PLM | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Room Tempreture Crystal Structure of Sterol Carrier Protein-2 Like-2
Overview
Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [(3)H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport.
About this Structure
3BDQ is a Single protein structure of sequence from Aedes aegypti. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:18084051
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