4qry: Difference between revisions
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''' | ==the ground state and the N intermediate of pharaonis halorhodopsin in complex with bromide ion== | ||
<StructureSection load='4qry' size='340' side='right' caption='[[4qry]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4qry]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QRY FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=L2P:2,3-DI-PHYTANYL-GLYCEROL'>L2P</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3a7k|3a7k]], [[3qbk|3qbk]], [[3qbg|3qbg]], [[3abw|3abw]], [[3vvk|3vvk]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qry OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qry RCSB], [http://www.ebi.ac.uk/pdbsum/4qry PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Halorhodopsin from Natronomonas pharaonis (pHR) functions as a light-driven halide ion pump. In the presence of halide ions, the photochemical reaction of pHR is described by the scheme: K--> L1 --> L2 --> N --> O --> pHR' --> pHR. Here, we report light-induced structural changes of the pHR-bromide complex observed in the C2 crystal. In the L1-to-L2 transition, the bromide ion that initially exists in the extracellular vicinity of retinal moves across the retinal Schiff base. Upon the formation of the N state with a bromide ion bound to the cytoplasmic vicinity of the retinal Schiff base, the cytoplasmic half of helix F moves outward to create a water channel in the cytoplasmic interhelical space, whereas the extracellular half of helix C moves inward. During the transition from N to an N-like reaction state with retinal assuming the 13-cis/15-syn configuration, the translocated bromide ion is released into the cytoplasmic medium. Subsequently, helix F relaxes into its original conformation, generating the O state. Anion uptake from the extracellular side occurs when helix C relaxes into its original conformation. These structural data provide insight into the structural basis of unidirectional anion transport. | |||
Crystal Structures of the L1, L2, N, and O States of pharaonis Halorhodopsin.,Kouyama T, Kawaguchi H, Nakanishi T, Kubo H, Murakami M Biophys J. 2015 Jun 2;108(11):2680-90. doi: 10.1016/j.bpj.2015.04.027. PMID:26039169<ref>PMID:26039169</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Natronomonas pharaonis]] | |||
[[Category: Kawaguchi, H]] | |||
[[Category: Kouyama, T]] | [[Category: Kouyama, T]] | ||
[[Category: | [[Category: Transmembrane helice]] | ||
[[Category: Light-driven chloride ion pump]] | |||
[[Category: Membrane]] | |||
[[Category: Membrane protein]] | |||
[[Category: Retinal bacterioruberin]] | |||
