3kvt: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:3kvt.jpg|left|200px]] | [[Image:3kvt.jpg|left|200px]] | ||
'''TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL''' | {{Structure | ||
|PDB= 3kvt |SIZE=350|CAPTION= <scene name='initialview01'>3kvt</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
3KVT is a [ | 3KVT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVT OCA]. | ||
==Reference== | ==Reference== | ||
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http:// | Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9886290 9886290] | ||
[[Category: Aplysia californica]] | [[Category: Aplysia californica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 35: | ||
[[Category: zinc-binding]] | [[Category: zinc-binding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:05:38 2008'' | ||
Revision as of 17:05, 20 March 2008
| |||||||||||||
| 3kvt, resolution 2.00Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | ZN | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL
Overview
The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.
About this Structure
3KVT is a Single protein structure of sequence from Aplysia californica. Full crystallographic information is available from OCA.
Reference
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:9886290
Page seeded by OCA on Thu Mar 20 19:05:38 2008
