3wh8

Crystal structure of GH1 beta-glucosidase Td2F2 isofagomine complex

spinqualitylabelsall models 3wh8, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wh8 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, IFM, NA, NHE Related: 3wh5, 3wh6, 3wh7 Activity: Beta-glucosidase, with EC number 3.2.1.21 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The beta-glucosidase encoded by the td2f2 gene was isolated from a compost microbial metagenomic library by functional screening. The protein was identified to be a member of the glycoside hydrolase family 1 and was overexpressed in Escherichia coli, purified, and biochemically characterized. The recombinant beta-glucosidase, Td2F2, exhibited enzymatic activity with beta-glycosidic substrates, with preferences for glucose, fucose, and galactose. Hydrolysis occurred at the nonreducing end and in an exo manner. The order of catalytic efficiency for glucodisaccharides and cellooligosaccharides was sophorose > cellotetraose > cellotriose > laminaribiose > cellobiose > cellopentaose > gentiobiose, respectively. Intriguingly, the p-nitrophenyl-beta-D-glucopyranoside hydrolysis activity of Td2F2 was activated by various monosaccharides and sugar alcohols. At a D-glucose concentration of 1000 mM, enzyme activity was 6.7-fold higher than that observed in the absence of D-glucose. With 31.3 mM D-glucose, Td2F2 catalyzed transglycosylation to generate sophorose, laminaribiose, cellobiose, and gentiobiose. Transglycosylation products were detected under all activated conditions, suggesting that the activity enhancement induced by monosaccharides and sugar alcohols may be due to the transglycosylation activity of the enzyme. These results show that Td2F2 obtained from a compost microbial metagenome may be a potent candidate for industrial applications. Characterization of a novel beta-glucosidase from a compost microbial metagenome with strong transglycosylation activity.,Uchiyama T, Miyazaki K, Yaoi K J Biol Chem. 2013 Jun 21;288(25):18325-34. doi: 10.1074/jbc.M113.471342. Epub, 2013 May 9. PMID:23661705 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-glucosidase

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