5by8: Difference between revisions
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''' | ==The structure of Rpf2-Rrs1 explains its role in ribosome biogenesis== | ||
<StructureSection load='5by8' size='340' side='right' caption='[[5by8]], [[Resolution|resolution]] 1.52Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5by8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BY8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BY8 FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5by8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5by8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5by8 RCSB], [http://www.ebi.ac.uk/pdbsum/5by8 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The assembly of eukaryotic ribosomes is a hierarchical process involving about 200 biogenesis factors and a series of remodeling steps. The 5S RNP consisting of the 5S rRNA, RpL5 and RpL11 is recruited at an early stage, but has to rearrange during maturation of the pre-60S ribosomal subunit. Rpf2 and Rrs1 have been implicated in 5S RNP biogenesis, but their precise role was unclear. Here, we present the crystal structure of the Rpf2-Rrs1 complex from Aspergillus nidulans at 1.5 A resolution and describe it as Brix domain of Rpf2 completed by Rrs1 to form two anticodon-binding domains with functionally important tails. Fitting the X-ray structure into the cryo-EM density of a previously described pre-60S particle correlates with biochemical data. The heterodimer forms specific contacts with the 5S rRNA, RpL5 and the biogenesis factor Rsa4. The flexible protein tails of Rpf2-Rrs1 localize to the central protuberance. Two helices in the Rrs1 C-terminal tail occupy a strategic position to block the rotation of 25S rRNA and the 5S RNP. Our data provide a structural model for 5S RNP recruitment to the pre-60S particle and explain why removal of Rpf2-Rrs1 is necessary for rearrangements to drive 60S maturation. | |||
The | The structure of Rpf2-Rrs1 explains its role in ribosome biogenesis.,Kharde S, Calvino FR, Gumiero A, Wild K, Sinning I Nucleic Acids Res. 2015 Jun 27. pii: gkv640. PMID:26117542<ref>PMID:26117542</ref> | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Calvino, F R]] | |||
[[Category: Gumiero, A]] | [[Category: Gumiero, A]] | ||
[[Category: Kharde, S]] | [[Category: Kharde, S]] | ||
[[Category: Sinning, I]] | [[Category: Sinning, I]] | ||
[[Category: Wild, K]] | |||
[[Category: 5s rnp]] | |||
[[Category: Biosynthetic protein]] | |||
[[Category: Brix domain]] | |||
[[Category: Protein complex]] | |||
[[Category: Protein-rna interaction]] | |||
[[Category: Ribosome biogenesis]] | |||
