1elv: Difference between revisions
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==Overview== | ==Overview== | ||
C1s is the highly specific modular serine protease that mediates the, proteolytic activity of the C1 complex and thereby triggers activation of, the complement cascade. The crystal structure of a catalytic fragment from, human C1s comprising the second complement control protein (CCP2) module, and the chymotrypsin-like serine protease (SP) domain has been determined, and refined to 1.7 A resolution. In the areas surrounding the active site, the SP structure reveals a restricted access to subsidiary substrate, binding sites that could be responsible for the narrow specificity of C1s., The ellipsoidal CCP2 module is oriented perpendicularly to the surface of, the SP domain. This arrangement is maintained through a rigid, module-domain interface involving intertwined proline- and ... | C1s is the highly specific modular serine protease that mediates the, proteolytic activity of the C1 complex and thereby triggers activation of, the complement cascade. The crystal structure of a catalytic fragment from, human C1s comprising the second complement control protein (CCP2) module, and the chymotrypsin-like serine protease (SP) domain has been determined, and refined to 1.7 A resolution. In the areas surrounding the active site, the SP structure reveals a restricted access to subsidiary substrate, binding sites that could be responsible for the narrow specificity of C1s., The ellipsoidal CCP2 module is oriented perpendicularly to the surface of, the SP domain. This arrangement is maintained through a rigid, module-domain interface involving intertwined proline- and tyrosine-rich, polypeptide segments. The relative orientation of SP and CCP2 is, consistent with the fact that the latter provides additional substrate, recognition sites for the C4 substrate. This structure provides a first, example of a CCP-SP assembly that is conserved in diverse extracellular, proteins. Its implications in the activation mechanism of C1 are, discussed. | ||
==About this Structure== | ==About this Structure== | ||
1ELV is a | 1ELV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and NES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Complement_subcomponent_C1s Complement subcomponent C1s], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.42 3.4.21.42] Structure known Active Site: CAT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ELV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: trypsin-like serin protease]] | [[Category: trypsin-like serin protease]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:30:00 2007'' | ||
