1uvp: Difference between revisions
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==Overview== | ==Overview== | ||
Two series of complete and highly redundant data sets were collected at, wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine, pancreatic elastase (PPE). Radiation damage to the sample was evaluated, qualitatively by inspecting consecutive difference electron density maps, during the course of the experiment. The nature of the radiation damage, was found to be identical at both wavelengths and was localized primarily, at the four disulfide bridges of PPE, the cadmium site and the two, methionine residues. For a quantitative examination of the radiation, damage, the decrease in the peak height of the cadmium ion in various, electron density maps was exploited. Again, no significant difference in, radiation damage between the two wavelengths was observed. This can be, . | Two series of complete and highly redundant data sets were collected at, wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine, pancreatic elastase (PPE). Radiation damage to the sample was evaluated, qualitatively by inspecting consecutive difference electron density maps, during the course of the experiment. The nature of the radiation damage, was found to be identical at both wavelengths and was localized primarily, at the four disulfide bridges of PPE, the cadmium site and the two, methionine residues. For a quantitative examination of the radiation, damage, the decrease in the peak height of the cadmium ion in various, electron density maps was exploited. Again, no significant difference in, radiation damage between the two wavelengths was observed. This can be, rationalized by considering the wavelength dependencies of the number of, diffracted photons versus the number of absorbed photons and the energy, deposited in the crystal by the latter. | ||
==About this Structure== | ==About this Structure== | ||
1UVP is a | 1UVP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with CD, ACT, CL and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UVP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:31:38 2007'' | ||
