1fhz: Difference between revisions
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|PDB= 1fhz |SIZE=350|CAPTION= <scene name='initialview01'>1fhz</scene>, resolution 2.20Å | |PDB= 1fhz |SIZE=350|CAPTION= <scene name='initialview01'>1fhz</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=PSO:4 | |LIGAND= <scene name='pdbligand=PSO:4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN'>PSO</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
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[[Category: psoralen]] | [[Category: psoralen]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 11:47:24 2008'' | ||
Revision as of 09:47, 23 March 2008
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| 1fhz, resolution 2.20Å | |||||||||||||
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| Ligands: | PSO | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION
Overview
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
About this Structure
1FHZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703
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