2bj1: Difference between revisions
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==Overview== | ==Overview== | ||
The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR), encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity, with Escherichia coli nickel responsive repressor NikR (EcNikR), including, many residues that are functionally important in the E.coli ortholog. We, succeeded in crystallization and structural characterization of PhNikR in, the apo form and two nickel bound forms that exhibit different, conformations, open and closed. Moreover, we have identified a putative, "low-affinity" nickel-binding pocket in the closed form. This binding site, has unusual nickel coordination and exhibits high sensitivity to phosphate, in the crystal structure. Analysis of the PhNikR structures and, structure-based mutational studies with EcNikR reveals a plausible, . | The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR), encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity, with Escherichia coli nickel responsive repressor NikR (EcNikR), including, many residues that are functionally important in the E.coli ortholog. We, succeeded in crystallization and structural characterization of PhNikR in, the apo form and two nickel bound forms that exhibit different, conformations, open and closed. Moreover, we have identified a putative, "low-affinity" nickel-binding pocket in the closed form. This binding site, has unusual nickel coordination and exhibits high sensitivity to phosphate, in the crystal structure. Analysis of the PhNikR structures and, structure-based mutational studies with EcNikR reveals a plausible, mechanism of nickel-dependent promoter recognition by the NikR family of, proteins. | ||
==About this Structure== | ==About this Structure== | ||
2BJ1 is a | 2BJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with NI and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJ1 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:32:22 2007'' | ||
