4v45

E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE

spinqualitylabelsall models 4v45, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v45 is a 16 chain structure. This structure supersedes and combines the now removed PDB entries 1jz0 and 1jz1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 2FG, MG, NA NonStd Res: CME Related: 1jz0, 1jz1, 1dp0, 1f49, 1jyn, 1jyv, 1jyw, 1jyx, 1jyy, 1jyz, 1jz2, 1jz3, 1jz4, 1jz5, 1jz6, 1jz7, 1jz8 Activity: Beta-galactosidase, with EC number 3.2.1.23 Resources: FirstGlance, OCA, RCSB, PDBsum Warning: this is a large structure, and loading might take a long time or not happen at all. Publication Abstract from PubMed The structures of a series of complexes designed to mimic intermediates along the reaction coordinate for beta-galactosidase are presented. These complexes clarify and enhance previous proposals regarding the catalytic mechanism. The nucleophile, Glu537, is seen to covalently bind to the galactosyl moiety. Of the two potential acids, Mg(2+) and Glu461, the latter is in better position to directly assist in leaving group departure, suggesting that the metal ion acts in a secondary role. A sodium ion plays a part in substrate binding by directly ligating the galactosyl 6-hydroxyl. The proposed reaction coordinate involves the movement of the galactosyl moiety deep into the active site pocket. For those ligands that do bind deeply there is an associated conformational change in which residues within loop 794-804 move up to 10 A closer to the site of binding. In some cases this can be inhibited by the binding of additional ligands. The resulting restricted access to the intermediate helps to explain why allolactose, the natural inducer for the lac operon, is the preferred product of transglycosylation. A structural view of the action of Escherichia coli (lacZ) beta-galactosidase.,Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW Biochemistry. 2001 Dec 11;40(49):14781-94. PMID:11732897 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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