4w5v: Difference between revisions

Line 1:

'''~~Unreleased structure~~'''

==Crystal structure of Human SUMO E2-conjugating enzyme (Ubc9) in complex with E1-activating enzyme (Uba2) ubiquitin fold domain (Ufd)==

~~The entry~~ 4w5v is ~~ON HOLD until Paper Publication~~

== Structural highlights ==

<table><tr><td colspan='2'>[[4w5v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W5V FirstGlance]. <br>

~~Authors~~: ~~Boucher, L~~.E., ~~Reiter~~, K.H., ~~Matunis~~, M.J., ~~Bosch, J~~.

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w5v RCSB], [http://www.ebi.ac.uk/pdbsum/4w5v PDBsum]</span></td></tr>

~~Description:~~

</table>

[[~~Category~~: ~~Unreleased Structures~~]]

== Function ==

[[~~Category~~: ~~Matunis, M~~.J]]

[[http://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN]] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref> [[http://www.uniprot.org/uniprot/SAE2_HUMAN SAE2_HUMAN]] The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.<ref>PMID:11481243</ref> <ref>PMID:11451954</ref> <ref>PMID:19443651</ref> <ref>PMID:15660128</ref> <ref>PMID:17643372</ref> <ref>PMID:20164921</ref>

~~[[Category: Boucher~~, L.~~E]]~~

== References ==

__TOC__

</StructureSection>

[[Category: Bosch, J]]

[[Category: Reiter, K.H]]

[[Category: Boucher, L E]]

[[Category: Matunis, M J]]

[[Category: Reiter, K H]]

[[Category: E1-conjugating enzyme]]

[[Category: E2-activating enzyme]]

[[Category: Ligase]]

[[Category: Sumo]]

[[Category: Uba2]]

[[Category: Ubc9]]

[[Category: Ubiquitin]]

[[Category: Ubiquitin fold domain]]

[[Category: Ufd]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Crystal structure of Human SUMO E2-conjugating enzyme (Ubc9) in complex with E1-activating enzyme (Uba2) ubiquitin fold domain (Ufd)==
+<StructureSection load='4w5v' size='340' side='right' caption='[[4w5v]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-The entry 4w5v is ON HOLD  until Paper Publication
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4w5v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W5V FirstGlance]. <br>
-Authors: Boucher, L.E., Reiter, K.H., Matunis, M.J., Bosch, J.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w5v RCSB], [http://www.ebi.ac.uk/pdbsum/4w5v PDBsum]</span></td></tr>
-Description:
+</table>
-[[Category: Unreleased Structures]]
+== Function ==
-[[Category: Matunis, M.J]]
+[[http://www.uniprot.org/uniprot/UBC9_HUMAN UBC9_HUMAN]] Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.<ref>PMID:8668529</ref> <ref>PMID:11451954</ref> <ref>PMID:15809060</ref> <ref>PMID:19744555</ref> <ref>PMID:19638400</ref> <ref>PMID:17466333</ref> <ref>PMID:20077568</ref>  [[http://www.uniprot.org/uniprot/SAE2_HUMAN SAE2_HUMAN]] The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.<ref>PMID:11481243</ref> <ref>PMID:11451954</ref> <ref>PMID:19443651</ref> <ref>PMID:15660128</ref> <ref>PMID:17643372</ref> <ref>PMID:20164921</ref>
-[[Category: Boucher, L.E]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bosch, J]]
-[[Category: Reiter, K.H]]
+[[Category: Boucher, L E]]
+[[Category: Matunis, M J]]
+[[Category: Reiter, K H]]
+[[Category: E1-conjugating enzyme]]
+[[Category: E2-activating enzyme]]
+[[Category: Ligase]]
+[[Category: Sumo]]
+[[Category: Uba2]]
+[[Category: Ubc9]]
+[[Category: Ubiquitin]]
+[[Category: Ubiquitin fold domain]]
+[[Category: Ufd]]