2k0a

1H, 15N and 13C chemical shift assignments for Rds3 protein

spinqualitylabelsall models 2k0a, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2k0a is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Gene: RDS3 (Saccharomyces cerevisiae) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [RDS3_YEAST] Required for pre-mRNA splicing. Involved in regulation of drug sensitivity and may play a role in multidrug resistance. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Rds3p, a component of the U2 snRNP subcomplex SF3b, is essential for pre-mRNA splicing and is extremely well conserved in all eukaryotic species. We report here the solution structure of Rds3p, which reveals an unusual knotted fold unrelated to previously known knotted proteins. Rds3p has a triangular shape with a GATA-like zinc finger at each vertex. Pairs of cysteines contributing to each finger are arranged nonsequentially in a permuted arrangement reminiscent of domain-swapping but which here involves segments of subdomains within a single chain. We suggest that the structure arose through a process of segment swapping after gene duplication. The fingers are connected through beta-strands and loops, forming an overall topology strongly resembling a "triquetra knot." The conservation and surface properties of Rds3p suggest that it functions as a platform for protein assembly within the multiprotein SF3b complex of U2 snRNP. The recombinant protein used for structure determination is biologically active, as it restores splicing activity in a yeast splicing extract depleted of native Rds3p. Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif.,van Roon AM, Loening NM, Obayashi E, Yang JC, Newman AJ, Hernandez H, Nagai K, Neuhaus D Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9621-6. Epub 2008 Jul 8. PMID:18621724 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pre-mRNA-splicing factor

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