1a54: Difference between revisions
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==Overview== | ==Overview== | ||
Crystal structures are presented for the A197C mutant of Escherichia coli, phosphate binding protein (PBP) and the same mutant labeled at Cys197 with, N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC)., Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic, phosphate. The resulting high-fluorescence state of the coumarin and the, ability of this coumarin to monitor the conformational changes associated, with inorganic phosphate binding are interpreted in terms of the specific, interactions of MDCC with the protein. The structure helps to explain why, this particular label gives a high-fluorescence state on binding inorganic, phosphate, while several other related labels do . | Crystal structures are presented for the A197C mutant of Escherichia coli, phosphate binding protein (PBP) and the same mutant labeled at Cys197 with, N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC)., Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic, phosphate. The resulting high-fluorescence state of the coumarin and the, ability of this coumarin to monitor the conformational changes associated, with inorganic phosphate binding are interpreted in terms of the specific, interactions of MDCC with the protein. The structure helps to explain why, this particular label gives a high-fluorescence state on binding inorganic, phosphate, while several other related labels do not, and hence aids our, general understanding of environmentally sensitive fluorescence probes on, proteins. | ||
==About this Structure== | ==About this Structure== | ||
1A54 is a | 1A54 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with 2HP and MDC as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Sites: 2HP and MDC. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A54 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:37:48 2007'' | ||
