1upn: Difference between revisions

Echovirus type 12 (EV12), an Enterovirus of the Picornaviridae family, uses the complement regulator decay-accelerating factor (DAF, CD55) as a, cellular receptor. We have calculated a three-dimensional reconstruction, of EV12 bound to a fragment of DAF consisting of short consensus repeat, domains 3 and 4 from cryo-negative stain electron microscopy data (EMD, code 1057). This shows that, as for an earlier reconstruction of the, related echovirus type 7 bound to DAF, attachment is not within the viral, canyon but occurs close to the 2-fold symmetry axes. Despite this general, similarity our reconstruction reveals a receptor interaction that is quite, different from that observed for EV7. Fitting of the crystallographic, co-ordinates for DAF(34) and EV11 into the reconstruction shows a close, agreement between the crystal structure of the receptor fragment and the, density for the virus-bound receptor, allowing unambiguous positioning of, the receptor with respect to the virion (PDB code 1UPN). Our finding that, the mode of virus-receptor interaction in EV12 is distinct from that seen, for EV7 raises interesting questions regarding the evolution and, biological significance of the DAF binding phenotype in these viruses.

1UPN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_echovirus_11 Human echovirus 11]. Structure known Active Site: RB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UPN OCA].  

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