1gnl: Difference between revisions

The structures of the hybrid cluster proteins (HCPs) from the, sulfate-reducing bacteria Desulfovibrio desulfuricans (ATCC 27774) and, Desulfovibrio vulgaris (Hildenborough) have been elucidated at a, resolution of 1.25 A using X-ray synchrotron radiation techniques. In the, case of the D. desulfuricans protein, protein isolation, purification, crystallization and X-ray data collection were carried out under strict, anaerobic conditions, whereas for the D. vulgaris protein the conditions, were aerobic. However, both structures are essentially the same, comprising three domains and two iron-sulfur centres. One of these centres, situated near the exterior of the molecules in domain 1 is a cubane, [4Fe-4S] cluster, whereas the other, located at the interface of the three, domains, contains the unusual four-iron cluster initially found in the D., vulgaris protein. Details of the structures and the associated EPR, spectroscopy of the D. desulfuricans protein are reported herein. These, structures show that the nature of the hybrid cluster, containing both, oxygen and sulfur bridges, is independent of the presence of oxygen in the, isolation and crystallization procedure and also does not vary, significantly with changes in the oxidation state. The structures and, amino acid sequences of the HCP are compared with the recently elucidated, structure of the catalytic subunit of a carbon monoxide dehydrogenase from, Carboxydothermus hydrogenoformans and related dehydrogenases. Electronic, supplementary material to this paper can be obtained by using the Springer, Link server located at http://dx.doi.org/10.1007/s00775-001-0326-y.

1GNL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with ACT, SF4 and FSO as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: F4A. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GNL OCA].  

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:40:48 2007''